Keywords
mesenchymal stem cells
peripheral nerves
How to Cite
Abstract
Abstract
EFFICACY OF FIBRIN MATRIX WITH NEUROINDUCED MESENCHYMAL STEM CELLS TRANSPLANTATION FOR RESTORATION SCIATIC NERVE FUNCTION AFTER ITS COMPLETE RUPTURE IN RATS
Piatykop V., Kaliuzhka V., Shchegelska O., Markevych M.
Peripheral nerves damage is a frequent pathology with significant socio-economic significance. The aim is to study the possibility of using fibrin matrices filled with neuroinduced mesenchymal stem cells (nMSC) to restore integrity of peripheral nerves. Methods. The study was carried out on 40 mongrel female rats. Sciatic nerves (SN) of all rats were intersected and then reconstituted using various methods. nMSC were obtained from rats` bone marrow and cultivated by special method. Results. Total anatomical rupture of SN without treatment led to persistent neurologic deficit (SFI = -98) in E1 group. Partial restoration of SN function increased to SFI = -37 on the 30th day in E2 (operative reconstruction) group. Partial restoration of SN function occurred after 20 days (SFI = -64) in E3 group (transplanted acellular fibrin matrix). Partial restoration of SN function started at the 3rd day, stably increased to SFI=-27 on 30th day in E4 group (transplanted fibrin matrix with nMSC). Histological evaluation showed: there were alternating portions of connective tissue with portions of nerve fibers in E2 group; in E3 group large scar was formed at the place of transplanted fibrin matrix; in E4 were found spindle-shaped and stellate cells with long processes running from one side of SN to another, cells of connective tissue and thin nerve fibers. Conclusions. It has been shown that transplantation of the fibrin matrix with nMSC was more effective for treatment of SN trauma than transplantation of cell-free fibrin matrix and close to the results of surgical reconstruction.
Keywords: mesenchymal stem cells, sciatic nerve, fibrin matrix.
Резюме
ЕФЕКТИВНІТЬ ТРАНСПЛАНТАЦІЇ ФІБРИНОВИХ МАТРИЦЬ ЗНЕЙРОІНДУКОВАНИМИ МЕЗЕНХІМАЛЬНИМИ СТОВБУРОВИМИ КЛІТИНАМИДЛЯ ВІДНОВЛЕННЯ ФУНКЦІЇ СІДНИЧНОГО НЕРВУ ПІСЛЯ ЙОГО РОЗРИВУ УЩУРІВ
П’ятикоп В.О., Калюжка В.Ю., Щегельська О.А., Маркевич М.А.
Пошкодження периферичних нервів є частою патологією, що має значне соціально-економічне значення. Метою роботи є вивчення можливості використання фібринових матриць, заповнених нейроиндукованими мезенхімальними стовбуровими клітинами (нМСК) для відновлення цілісності периферичних нервів. Методи. Дослідження проводили на 40 беспородних щурах. Сідничні нерви (СН) всіх щурів перетинали і потім відновлювали їх цілісність різними методами. МСК отримували з кісткового мозку щурів і культивували спеціальним методом.Результати. Загальний анатомічний розрив СН без лікування призводив до стійкого неврологічного дефіциту (Sciatic functional index (SFI) = -98) у групі Е1. Часткове відновлення функції СН зросло до SFI = -37 на 30-й день у групі E2 (оперативна реконструкція). Часткове відновлення функції СН відбувалося через 20 днів (SFI = -64) в групі Е3 (трансплантований фібриновий безклітинний матрикс). Часткове відновлення функції SN починалося на 3-й день, стабільно збільшувалося до SFI=-27 на 30-й день у групі E4 (трансплантований фібриновий матрикс з нМСК). Гістологічна оцінка показала: формувались ділянки сполучної тканини з ділянками нервових волокон у групі Е2; у групі Е3 великий сполучнотканинниий рубець утворився на місці трансплантованого фібринового матриксу; в Е4 були виявлені веретеноподібні і зірчасті клітини з довгими відросткамии від однієї сторони СН до іншої, клітини сполучної тканини і тонкі нервові волокна. Висновки. Було показано, що трансплантація фібринового матриксу з нМСК була більш ефективною для лікування травми СН, ніж трансплантація безклітинної фібринової матриці та близька до результатів хірургічної реконструкції.
Ключові слові: мезенхімальні стовбурові клітини, сідничний нерв, фібринова матриця.
Резюме.
ЭФЕКТИВНОСТЬ ТРАНСПЛАНТАЦИИ ФИБРИНОВЫХ МАТРИЦ С НЕЙРОИНДУЦИРОВАННЫМИ МЕЗЕНХИМАЛЬНЫМИ СТВОЛОВЫМИ КЛЕТКАМИ ДЛЯ ВОССТАНОВЛЕНИЯ ФУНКЦИИ СЕДАЛИЩНОГО НЕРВА ПОСЛЕ ЕГО РАЗРЫВА У КРЫС.
Пятикоп В.А., Калюжка В.Ю., Щегельская Е.А., Маркевич Н.А.
Повреждение периферических нервов является частой патологией, имеющей значительное социально-экономическое значение. Цель состоит в том, чтобы изучить возможность использования фибриновых матриц, заполненных нейроиндуцированными мезенхимальными стволовыми клетками (нМСК), для восстановления целостности периферических нервов. Методы. Исследование проведено на 40 беспородных самках крыс. Седалищные нервы (СН) всех крыс пересекали и затем восстанавливали, используя различные методы. нМСК получали из костного мозга крыс и культивировали специальным способом. Результаты. Полный анатомический разрыв СН без лечения привел к стойкому неврологическому дефициту (SFI = -98) в группе Е1. Частичное восстановление функции SN увеличилось до SFI = -37 на 30-й день в группе E2 (оперативная реконструкция). Частичное восстановление функции СН произошло через 20 дней (SFI = -64) в группе E3 (трансплантированный фибриновый бесклеточный матрикс). Частичное восстановление функции СН началось на 3-й день, стабильно увеличилось до SFI = -27 на 30-й день в группе Е4 (трансплантированный фибриновый матрикс с нМСК). Гистологическая оценка показала: в группе Е2 были чередующиеся участки соединительной ткани с нервными волокнами; в группе Е3 образовался крупный рубец на месте трансплантированного фибринового матрикса; в E4 были обнаружены веретенообразные и звездчатые клетки с длинными отростками, идущими от одной стороны СН к другой, клетками соединительной ткани и тонкими нервными волокнами. Выводы. Было показано, что трансплантация фибринового матрикса с нМСК была более эффективной для лечения травмы СН, чем трансплантация бесклеточного фибринового матрикса и близка к результатам хирургической реконструкции.
Ключевые слова: мезенхимальные стволовые клетки, седалищный нерв, фибриновая матрица
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