Abstract
SUMMARY
Yu.V. Odynets, , Y.V.Vasylchenko
THE ROLE OF VASCULAR INTERCELLULAR ADHESION MOLECULE-1 (sVCAM-1) IN INFLAMMATION DEVELOPMENT ACCOMPANYING BRONCHIAL ASTHMA IN CHILDREN
Kharkiv National Medical University, Ukraine
Abstract. The goal of the investigation is to evaluate the role of sVCAM-1 in inflammation development in children suffering from bronchial asthma at the period of exacerbations and remission. The study included 91 patients with persistent bronchial asthma at the age of 6 to 17 years. The serum levels of sVCAM-1 was determined with enzyme-linked immunosorbent assay. Intima media complex thickness of common carotid artery was detected applying to the method of Pignolli P. (1986). Clinical investigations of blood were performed according to common methods. Statistic analysis was performed using Statistics packages such as “EXCELL FOR WINDOWS” and “STATISTICA 7.0. FOR WINDOWS”. The study of sVCAM-1 level in blood serum of children suffering from BA showed its significant increase in mentioned patients both at the exacerbation and remission periods, and it depends on disease severity; its decrease was reported at remission period. sVCAM-1 was found to directly participate in inflammatory cells adhesion processes (neutrophils, eosinophils, and monocytes) on vascular endothelium with their further migration not only at the period of BA exacerbation but also at remission period. It leads to development of local inflammation and thickening of vascular wall. The present correlations with external respiration function suggest direct participation of sVCAM-1 in development of endothelial dysfunction and severity of BA manifestation.
Keywords: bronchial asthma, children, inflammation.
Анотація
Ю.В.Одинець, Ю.В.Васильченко
Роль судинної молекули міжклітинної адгезії-1 (sVCAM-1) в розвитку запалення при бронхіальній астмі у дітей.
Харківський національний медичний університет, Україна
Резюме. Метою дослідження є оцінити роль sVCAM-1 у формуванні запалення при бронхіальній астмі у дітей в періодах загострення та ремісії.
У дослідження включено 91 дитина з персистуючою бронхіальною астмою у віці від 6 до 17 років. Для досягнення мети дослідження визначали рівень sVCAM-1 в сироватці крові імуноферментним методом; товщину комплексу інтими-медіа загальної сонної артерії – за Pignolli P. (1986). Клінічні дослідження крові проводили загальноприйнятими методами. Статистичний аналіз здійснювали за допомогою статистичних пакетів "EXCELL FOR WINDOWS" і "STATISTICA 7.0. FOR WINDOWS". При вивченні рівня sVCAM-1 в сироватці крові у дітей хворих на БА встановлено достовірне підвищення його у вказаних пацієнтів як в періоді загострення, так і в періоді ремісії, та залежить від тяжкості захворювання; відзначено його зниження в періоді ремісії. Зазначена безпосередня участь sVCAM-1 в процесах адгезії клітин запалення (нейтрофілів, еозинофілів, моноцитів) на ендотелії судини з наступною їх трансендотеліальною міграцією не тільки в періоді загострення БА, а і в періоді ремісії. Це призводить до розвитку місцевого запалення, а отже і потовщення стінки судини. Наявні взаємозв’язки з показниками ФЗД свідчать про безпосередню причатність sVCAM-1 в розвитку ендотеліальної дисфункції та тяжкості прояву БА.
Ключові слова: бронхіальна астма, діти, запалення.
Аннотация
Ю.В.Одинец, Ю.В.Васильченко
Роль сосудистой молекулы межклеточной адгезии-1 (sVCAM-1) в развитии воспаления при бронхиальной астме у детей.
Целью исследования является оценить роль sVCAM-1 в формировании воспаления при бронхиальной астме у детей в период обострения и ремиссии. В исследование включен 91 ребенок с персистирующей бронхиальной астмой в возрасте от 6 до 17 лет. Для достижения цели исследования определяли уровень sVCAM-1 в сыворотке крови иммуноферментным методом; толщину комплекса интима-медиа общей сонной артерии – по методу Pignolli P. (1986). Клинические исследования крови проводили общепринятыми методами. Статистический анализ осуществляли с помощью статистических пакетов "EXCELL FOR WINDOWS" и "STATISTICA 7.0. FOR WINDOWS". При изучении уровня sVCAM-1 в сыворотке крови у детей больных БА установлено достоверное повышение его у указанных пациентов как в периоде обострения, так и в периоде ремиссии, и зависит от тяжести заболевания; отмечено снижение уровня sVCAM-1 в сыворотке крови в периоде ремиссии. Показано непосредственное участие sVCAM-1 в процессах адгезии клеток воспаления (нейтрофилов, эозинофилов, моноцитов) на эндотелии сосуда с последующей их трансэндотелиальных миграцией не только в период обострения БА, но и в периоде ремиссии. Это приводит к развитию местного воспаления, а следовательно и утолщение стенки сосуда. Имеющиеся взаимосвязи с показателями функции внешнего дыхания свидетельствуют о непосредственной причатности sVCAM-1 в развитии эндотелиальной дисфункции и тяжести проявления БА.
Ключевые слова: бронхиальная астма, дети, воспаление.
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