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Abstract
CONNECTIVE TISSUE METABOLISM IN PATIENTS WITH JUVENILE IDIOPATHIC ARTHRITIS: 10-YEAR FOLLOW-UP STUDY
Shevchenko N.S., Panko N.O., Rakovska L.O., Holovko T.O.
Connective tissue remodeling is essential for progressive course of juvenile idiopathic arthritis (JIA), therefore changes in glucosaminoglycans (GAGs) fractions with time in patients with JIA have been studied during 10-year follow-up. The study involved 22 healthy children (controls) and 83 patients with JIA aged from 2 to 18. The follow‑up study was yearly held for 10 years and initially included 14 patients with JIA investigated for connective tissue metabolism.
Proteoglycans metabolism in children with JIA is characterized by a decrease in the total GAGs, mainly by means of low levels of chondroitin-4-sulfate and highly sulphonated GAGs. This redistribution was more specific for children with prolonged disease duration (more than 5 years). Slowing of proteoglycans metabolism in reducing the excretion of uronic acids was the most common for up to 2 years of disease duration as compared to later stages. The severity of metabolic imbalance of proteoglycans depends on the duration of JIA and has the most unfavorable period of 5 years and later after the onset of the disease.
Keywords: juvenile idiopathic arthritis, connective tissue metabolism.
МЕТАБОЛІЗМ СПОЛУЧНОЇ ТКАНИНИ У ПАЦІЄНТІВ ІЗ ЮВЕНІЛЬНИМ ІДІОПАТИЧНИМ АРТРИТОМ: 10-РІЧНИЙ КАТАМНЕЗ.
Шевченко Н.С., Панько Н.О., Раковська Л.О., Головко Т.О.
Ремоделювання сполучної тканини є важливою складовою прогресуючого перебігу ювенільного ідіопатичного артриту (ЮІА), тому метою даного дослідження було вивчення динаміки змін з боку окремих фракцій глюкозаміногліканів (ГАГ) у пацієнтів з ЮІА впродовж 10 років. Дослідження включало 83 пацієнта з ЮІА віком від 2 до 18 років і 22 здорові дитини порівнюваних за віком та статтю. Катамнестичне спостереження проводилося протягом 10 років і спочатку стартувало з обстеження сполучнотканинного обміну у 14 хворих на ЮІА, показники якого у подальшому контролювалися в динаміці.
В результаті дослідження було виявлено, що метаболізм протеогліканів у дітей з ЮІА характеризувався зниженням загального рівня ГАГ, в основному за рахунок хондроїтин-4-сульфату і високосульфанізованих ГАГ. Дана тенденція була найбільш характерною для доволі пізніх строків розвитку ЮІА (при анамнезі більше 5 років). Уповільнення обміну протеогліканів, про що свідчило зниження екскреції уронових кислот, найбільш часто виявлялося в період до 2 років у порівнянні з більш пізніми термінами перебігу хвороби.Таким чином, зміни сполучнотканинного обміну залежали від тривалості захворювання і були найбільш виражені в період 5 років і більше від початку захворювання.
Ключові слова: ювенільний ідіопатичний артрит, обмін сполучної тканини.
МЕТАБОЛИЗМ СОЕДИНИТЕЛЬНОЙ ТКАНИ У ПАЦИЕНТОВ С ЮВЕНИЛЬНЫМ ИДИОПАТИЧЕСКИМ АРТРИТОМ: 10-ЛЕТНИЙ КАТАМНЕЗ.
Шевченко Н.С., Панько Н.А., Раковская Л.А., Головко Т.А.
Ремоделирование соединительной ткани является важной составляющей прогрессирующего течения ювенильного идиопатического артрита (ЮИА), поэтому целью данного исследования было изучение динамики изменений со стороны отдельных фракций глюкозаминогликанов (ГАГ) у пациентов с ЮИА на протяжении 10 лет.
Исследование включало 83 пациента с ЮИА возрастом от 2 до 18 лет и 22 здоровых ребенка сопоставимых по возрасту и полу. Катамнестическое наблюдение проводилось в течение 10 лет и изначально стартовало с обследования соединительно-тканного обмена у 14 детей с ЮИА, который в последующем контролировался в динамике.
В результате исследования было выявлено, что метаболизм протеогликанов у детей с ЮИА характеризуется снижением общего уровня ГАГ, в основном за счет хондроитин-4-сульфата и высокосульфанизированных ГАГ. Данная тенденция была наиболее характерной для более позних сроков ЮИА (при анамнезе более 5 лет). Замедление обмена протеогликанов, о чем свидетельствовало снижение экскреции уроновых кислот, наиболее часто выявлялось в период до 2 лет в сравнении с более поздними сроками развития болезни.
Таким образом, изменения соединительно-тканного обмена зависели от длительности заболевания и были наиболее выражены в период 5 лет и более от начала заболевания.
Ключевые слова: ювенильный идиопатический артрит, обмен соединительной ткани.
References
Aspberg, A. (2012). The different roles of aggrecan interaction domains. Journal of Histochemistry & Cytochemistry, 60(12), 987-996. doi:10.1369/0022155412464376
Frantz, C., Stewart, K. M., Weaver, V. M. (2010). The extracellular matrix at a glance. Journal of Cell Science, 123, 4195-4200. doi:10.1242/jcs.023820.
Горячковский, A. M. (2005) Клиническая биохимия в лабораторной диагностике. Одесса: Экология. [Goryachkovskiy A. M. (2005) Klinicheskaya biokhimiya v laboratornoy diagnostike. Odessa: Ekologiya].
Hanyecz, A., Olasz, K., Tarjanyi, O., Nemeth, P., Mikecz, K., Glant, T. T., Boldizsar, F. (29 January 2014). Proteoglycan aggrecan conducting T cell activation and apoptosis in a murine model of rheumatoid arthritis. Biomed Research International, Volume 2014, Article ID 942148, 13 pages. http://dx.doi.org/10.1155/2014/942148
Jura-Półtorak, A., Komosinska-Vassev, K., Kotulska, A., Kucharz, E. J., Klimek, K., Kopec-Medrek, M., Olczyk, K. (10 June 2014) Alterations of plasma glycosaminoglycan profile in patients with rheumatoid arthritis in relation to disease activity. Clinica chimica acta, Volume 433, 20-27. doi:10.1016/j.cca.2014.02.027.
Kim, H. K., Laor, T., Graham, T. B., Anton, C. G., Salisbury, S. R., Racadio, J. M., Dardzinski, B. J. (2010). T2 relaxation time changes in distal femoral articular cartilage in children with juvenile idiopathic arthritis: a 3-year longitudinal study. American Journal of Roentgenology, 195(4), 1021-1025. doi:10.2214/AJR.09.4019.
Roughley, P. J., Mort, J. M. (2014). The role of aggrecan in normal and osteoarthritic cartilage. Journal of Experimental Orthopaedics, 1(8). doi:10.1186/s40634-014-0008-7.
Shevchenko, N. S., Lebec, I. S., Kashkalda, D. A. (2016) Changes of connective tissue biochemical indexes in children and adolescents with inflammatory diseases of joints. The Journal of V.N. Karazin Kharkiv National University, Series: biology, Issue 26, 11-15.
Штерн, М. Р., Тимошенко, О. П., Леонтьева, Ф. С., Клюева, Г. Ф. Способ определения глюкозаминогликанов в сыворотке крови. Патент A.s. 960626 SSSR, M. Kl 3.G 0,1 № 33148. SSSR. − №2998857128 − 13, Бюллетень № 35, 2 с. [Shtern M.P., Timoshenko O.P., Leontyeva F.S., Klyueva G.F. Sposob opredeleniya glikozaminoglikanov v syvorotke krovi. A.s. 960626 SSSR, M. Kl 3.G 0,1 № 33148. SSSR. − №2998857128 − 13; Zayavl. 23.10.80. Opubl. 23.09.82. Byul. №35. 2s.]
Struglics, A., Lohmander, L. S., Last, K., Akikusa, J., Allen, R., Fosang, A. J. (08 August 2012). Aggrecanase cleavage in juvenile idiopathic arthritis patients is minimally detected in the aggrecaninterglobular domain but robust at the aggrecan C-terminus. Arthritis & Rheumatism, Volume 64, Issue 12, 4151-4161. https://doi.org/10.1002/art.34665.
Theocharis, A. D., Skandalis, S. S., Gialeli, C., Karamanos, N. K. (1 February 2016). Extracellular matrix structure. Advanced Drug Delivery Reviews, Volume 97, 4–27. http://dx.doi.org/10.1016/j.addr.2015.11.001.
Winsz-Szczotka, K., Komosińska-Vassev, K., Kuźnik-Trocha, K., Gruenpeter, A., Lachór-Motyka, I., Olczyk, K. (June 2014) Influence of proteolytic-antiproteolytic enzymes and prooxidative-antioxidative factors on proteoglycan alterations in children with juvenile idiopathic arthritis. Clinical Biochemistry, Volume 47, Issue 9, 829-834. https://doi.org/10.1016/j.clinbiochem.2014.01.027.
Winsz-Szczotka, K., Komosińska-Vassev, K., Kuźnik-Trocha, K., Jura-PóBtorak, A. (January 2016). Laboratory Indicators of Aggrecan Turnover in Juvenile Idiopathic Arthritis. Disease Markers, Volume 2016, Article ID 7157169, 7 pages. https://www.hindawi.com/journals/dm/2016/7157169/.
Winsz-Szczotka, K., Komosińska-Vassev, K., Kuźnik-Trocha, K., Siwiec, A., Żegleń, B., Olczyk, K. (2015). Circulating keratan sulfate as a marker of metabolic changes of cartilage proteoglycan in juvenile idiopathic arthritis; influence of growth factors as well as proteolytic and prooxidative agents on aggrecan alterations. Clinical Chemistry and Laboratory Medicine, 53(2): 291-297. doi:10.1515/cclm-2014-0441.
Winsz-Szczotka, K., Mencner1, L., Olczyk, K. (2016). Metabolism of glycosaminoglycans in the course of juvenile idiopathic arthritis. Advances in Hygiene and Experimental Medicine, 70, 135-142. doi:10.5604/17322693.1196355.
Winsz-Szczotka, K., Kuźnik-Trocha, K., Komosińska-Vassev, K., Wisowski, G., Gruenpeter, A., Lachór-Motyka, I., Żegleń, B., Lemski, W., Olczyk, K. (2015). Plasma and urinary glycosaminoglycans in the course of juvenile idiopathic arthritis. Biochemical and Biophysical Research Communications, 458(3), 639-643. doi:10.1016/j.bbrc.2015.02.018.
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