Keywords
мікроорганізми, біоплівки, захворювання органів дихання, діти.
микроорганизмы, биопленки, заболевания органов дыхания, дети.
How to Cite
Abstract
DISTRIBUTION OF THE CAUSATIVE AGENTS OF RESPIRATORY TRACT INFECTIONS IN CHILDREN.
Mishyna М., Gonchar M., Logvinova O., Isaieva H., Basiuk M.
The study aimed to investigate prevalence of microorganisms depending on the site of isolation and disease. The study involved 48 children aged 1 year to 17 years. Acute bronchitis (54, 17%), community-acquired pneumonia (CAP) (33, 33%), bronchial asthma (12, 50%) were diagnosed. Were isolated 173 strains of microorganisms. Gram-positive microorganisms were detected 106 strains (61, 3%), Gram-negative microorganisms - 49 strains (28, 3%), fungi - 18 strains (10, 4%). We investigated 100 samples from nose (nasal swabs), pharynx (throat swabs) and sputum. In 83 cases were isolated Gram-positive microorganisms, in 36 cases were isolated Gram-negative microorganisms, in 18 cases - fungi. Analysis reviled that Staphylococcus aureus most often isolated from patients with acute bronchitis; Gram-negative microorganisms most often detected from throat swabs, comparing with microorganisms detected from nose swabs and sputum.
Keywords: microorganisms, biofilms, respiratory diseases, children.
Анотація
ПОШИРЕНІСТЬ ЗБУДНИКІВ ІНФЕКЦІЙ ДИХАЛЬНИХ ШЛЯХІВ У ДІТЕЙ.
Мішина М.М, Гончарь М. О., Логвінова О.Л., Ісаєва Г.О., Басюк М.А.
Метою дослідження було вивчити переважання умовно-патогенних мікроорганізмів, які викликають захворювання органів дихання у дітей, в залежності від місця виділення та захворювання. У дослідженні було 48 дітей у віці від 1 року до 17 років. Пацієнти були з такими діагнозами: гострі бронхіти (54, 17%), негоспітальні пневмонії (33, 33%), бронхіальна астма (12, 50%). Було виділено 173 штама умовно-патогенних мікроорганізмів. Грампозитивних мікроорганізмів було виділено 106 штамів (61, 3%), грамнегативних мікроорганізмів – 49 штамів (28, 3%), грибів – 18 штамів (10, 4%). Було досліджено 100 зразків з зіву, носу, мокротиння. Грампозитивні мікроорганізми були виділені з 83 зразків, грамнегативні – з 36 зразків, гриби – з 18 зразків. Проведене дослідження довело, що Staphylococcus aureus найчастіше виділявся у пацієнтів з гострими бронхітами. Грамнегативні мікроорганізми частіш за все виділялись зі зразків із зіву в порівнянні з мазками з носу та мокротинням.
Ключові слова: мікроорганізми, біоплівки, захворювання органів дихання, діти.
Абстракт
РАСПРОСТРАНЕННОСТЬ ВОЗБУДИТЕЛЕЙ ИНФЕКЦИЙ ДЫХАТЕЛЬНЫХ ПУТЕЙ У ДЕТЕЙ.
Мішина М.М., Гончарь М. О., Логвінова О.Л., Ісаєва Г.О., Басюк М.А.
Целью исследования было изучить преобладание условно-патогенныхмикроорганизмов, вызывающих заболевания органов дыхания у детей, в зависимости от места забора материала и заболевания. Исследование включало 48 детей в возрасте от 1 года до 17 лет. Пациенты находились с такими заболеваниями: острые бронхиты (54, 17%), внегоспитальные пневмонии (33, 33%), бронхиальная астма (12, 50%). Всего было выделено 173 штамма условно-патогенных микроорганизмов. Грамположительных микроорганизмов было выделено 106 штаммов (61, 3%), грамотрицательных микроорганизмов – 49 штаммов (28, 3%), грибов – 18 штаммов (10, 4%). Было исследовано 100 образцов из зева, носа, мокроты. Грамположительные микроорганизмы были выделены из 83 образцов, грамотрицальные – из 36 образцов, грибы – из 18 образцов. В ходе исследования было доказано, что Staphylococcus aureus чаще всего выделялся от пациентов с острыми бронхитами. Грамотрицательные микроорганизмы чаще всего выделялись из мазков из зева по сравнению с мазками из носа и мокротой.
Ключевые слова: микроорганизмы, биопленки, заболевания органов дыхания, дети.
References
Liu L., Johnson H.L., Cousens S., Perin J., Scott S., Lawn J.E., Rudan I., Campbell H., … Black R.E., (2012). Global, regional, and national cases of child mortality: an update systemic analysis for 2010 with time trends since 2000. Lancet, 379(9832), 2151-2161. doi: 10.1016/S0140-6736
http://dx.doi.org/10.1016/S0140-6736
Koppen I., Bosch A., Sanders E., van Houten M., Bogaert D., (2015). The respiratory microbiota during health and disease: a paediatric perspective. pneumonia, 6(1), 90-100. doi:10.1572/pneu.2015.6/656
Sakwinska O., Bastic Schmid V., Berger B., Bruttin A., Keitek K., Lepage M., et al. (2014) Nasopharyngeal microbiota in healthy children and pneumonia patients. Journal of Clinical Microbiology 52(5):1590- 1594. doi:10.1128/JCM.03280-13 http://dx.doi.org/10.1128/JCM.03280-13
Charalambous B.M., Leung M.H. (2012) Pneumococcal sepsis and nasopharyngeal carriage. Current opinion in pulmonary medicine.18(3) 222-227
https://doi.org/10.1097/MCP.0b013e328352103b.
Marks L.P., Reddinger R.M., Hakansson A.P., (2012) High levels of genetic recombination during nasopharyngeal carriage and biofilm formation in Streptococcus pneumonia. mBio 3(5), e0200-12. doi: 10.1128/mBio.0200-12
https://doi.org/10.1128/mBio.00200-12.
Marks L.R., Reddinger R.M., Hakansoon A.P. (2014) Biofilm formation enhances fomite survival of Streptococcus pneumonia and Streptococcus pyogenes. Infection and immunity 82(3), 1141-1146. doi:10.1128/IAI.01310-13
https://doi.org/10.1128/IAI.01310-13.
Reddinger R.M., Luke-Marshall N.R., Sauberan S.L., Hakansson A.P., Campagnaria A.A., (2018) Streptococcus pneumonia modulates Staphylococcus aureus biofilm dispersion and the transmission from colonization to invasive disease. mBio 9(1), e02089-17.
van den Bergh M.R., Biesbroek G., Rossen J.W., de Steenhuijsen Piters W.A., Bosch A.A., van Gils E. J., … Sanders E.A., (2012). Association between pathogens in the upper respiratory tract of young children: interplay between viruses and bacteria. PLoS One, 7(10), e47711
https://doi.org/10.1371/journal.pone.0047711.
Bosch A.A., Biesbroek A.A., Trzcinski K., Sanders E.A., Bogaert D., (2013). Viral and bacterial interactions in the upper respiratory tract. PLoS One, 9(1), e1003057. doi:10.1371/journal.ppat.1003057
https://doi.org/10.1371/journal.ppat.1003057.
Wertheim H.F., Vos M.C., Ott A., van Belkum A., Voss A., Kluytmans J.A., van Keulen P.H., Vandenbroucke-Grauls C.M., … Verbrugh H.A., (2004). Risk and outcome of nosocomial Staphylococcus aureus bacteraemia in nasal carriers versus non-carriers. Lancet. 364(9435), 703-705. doi: 10.1016/S0140-6736(04)16897-9 https://doi.org/10.1016/S0140-6736(04)16897-9.
Esposito S., Terranova L., Zampiero A., Ierardi V., Rios W.P., Pelucchi C., Principi N., (2014). Oropharyngeal and nasal Staphylococcus aureus carriage by healthy children. BMC Infectious Diseases.14:723. doi:10.1186/s12879-014-0723-9. https://doi.org/10.1186/s12879-014-0723-9.
Soares A.C., Souza D.G., Pinho V., Vieira A.T., Nicoli J.R., Cunha F.G., Mantovani A., Reis L.F., … Teixeira M.M., (2006). Dual function of the long pentraxin PTX3 in resistance against pulmonary infection with Klebsiella pneumonia in transgenic mice. Microbes and infection.8(5).
doi:10.1016/j.micinf.2005.12.017
Zhang P., Summer W.R., Bagby G.J., Nelson S., (2000). Innate immunity and pulmonary host defense. Immunological reviews.173, 39-51. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/10719666
Deep A., Chaudhary U., Gupta V., (2011). Quorum sensing and bacterial pathogenicity: from molecules to disease. Journal of Laboratory Physicians, 3(1), 4-11. doi:10.4103/0974-2727.78553
Byrd M.S., Bing P., Wenzhou H., Elizabeth A., Richard A., Chelsie E., Kristen E.D., Kyle M., … Swords E., (2011). Direct evaluation of Pseudomonas aeruginosa biofilm mediators in a chronic infection model. Infection and Immunity, 79(8), 3087-3095. doi:10.1128/IAI.00057-11
von Rosenvinge E.C., O’May G.A., Macfarlane S., Macfarlane G.T., Shirtliff M.E., (2013). Microbial biofilms and gastrointestinal diseases. Pathogens and disease, 67(1), 25-38. doi: 10.1111/2049-632X.12020
Foreman A., Jervis-Bardy J., Wormald P.J., (2011). Do biofilms contribute to the initiation and recalcitrance of chronic rhinosinusitis? The Laryngoscope, 121(5), 1085-1091. doi: 10.1002/lary.21438. http://dx.doi.org/10.1002/lary.21438.
Nickel J.C., Ruseska I., Wright J.B., Costerton J.W., (1985). Tobramycin resistance of Pseudomonas aeruginosa cell growing as a biofilm on urinary catheter material. Antimicrobial Agents and Chemotherapy, 27(4), 619-624. doi:10.1128/aac.27.4.619
Elaine M.M., Michael O.W., Lauren O.B., (2016). Type IV pilus expression in upregulated in Nontypeable Haemophilus influenzae biofilms formed at the temperature of the human nasopharynx. Journal of Bacteriology, 198(19), 2619-2630. doi:10.1128/JB.01022-15
Zar H.J., Tannenbaum E., Hanslo D., Hussey J., (2003). Sputum induction as a diagnostic tool for community-acquired pneumonia in infants and young children from a high HIV prevalence area. Pediatric Pulmonology, 3(6). doi:10.1002/ppul.10302
Lahti E., Peltola V., Waris M., Virkki R., Rantakokko-Jalava k., Jalava J., Eerola E., Ruuskanen O., (2009). Induced sputum in the diagnosis of childhood community-acquired pneumonia. Thorax, 64(3), 252-257. doi:10.1136/thx.2008.099051
Theophilus K.Adiku, Richard H.Asmah, Onike R., Bamenla G., Evangeline O., Andrew A.Adjei, Eric S.Donkor, George A., (2015). Aethiology of acute lower respiratory infections among children under five years in Accra, Ghana. Pathogens, 4(1), 22-33. doi:10.3390/pathogens4010022
El Seify M.Y., Fouda E.M., Ibrahim H.M., Fathy M.M., Husseiny Ahmed A.A., Khater W.S., El Deen N.N.Abouzeid H.G.,… Elbanna H.S., (2016). Microbial ethiology of community-acquired pneumonia among infants and children admitted to the pediatric hospital, Ain Shams University. European Journal of Microbiology and Immunology, 6(3), 206-214. doi:10.1556/1886.2016.00022.
Bhuyan G.S., Hossain M.A., Sarker S.K., Rahat A., Islam T., Haque T.N., Begum N., Qadri S.K., … Mannoor K., (2017). Bacterial and viral pathogen spectra of acute respiratory infections in under-5 children in hospital setting in Dhaka city. PLoS One, 12(3), e0174488 doi:10.1371/journal.pone.0174488
Hall-Stoodley L., Nistico L., Sambanthamoorthy K., Dice B., Nguyen D., Mershon W.J., Hu F.Z., Stoodley P., …Post J.C., (2008). Characterization of biofilm matrix, degradation by DNase treatment and evidence of capsule downregulation in Streptococcus pneumoniae clinical isolates. BMC Microbiology.8:173. doi:10.1186/1471-2180-8-173
Gilley R.P., Orithuela C.J., (2014). Pneumococci in biofilms are non-invasive: implications on nasopharyngeal colonization. Frontiers in Cellular and Infection Microbiology, 4:163. doi:10.3389/fcimb.2014.00163
https://doi.org/10.3389/fcimb.2014.00163.
Chao Y., Marks L.R., Pettigrew M.M., Hakansson A.P., (2014). Streptococcus pneumoniae biofilm formation and dispersion during colonization and disease. Frontiers in Cellular and Infection Microbiology,4:194. doi:10.3389/fcimb.2014.000194 https://doi.org/10.3389/fcimb.2014.00194.
Shak J.R., Vidal J.E., Klugman K.P., (2013). Influence on bacterial interactions on pneumococcal colonization of the nasopharynx. Trends in microbiology, 21(3), 129-135. doi:10.1016/j.tim.2012.11.005 https://doi.org/10.1016/j.tim.2012.11.005.
Simell B., Auranen K., Käyhty H., Goldblatt D., Dagan R., O’Brien K.L., (2012). The fundamental link between pneumococcal carriage and disease. Expert Reviews of Vaccines, 11(7), 841-855. doi:10.1586/erv.12.53
https://doi.org/10.1586/erv.12.53.
Wu X., Jacobs N.T., Boizo C., Palm P., Lattar S.M., Hanke C.R., Watson D.M., Sakai F., … Vidala J.E., (2017). Competitive dominance within biofilm consortia regulates the relative distribution of pneumococcal nasopharyngeal density. Applied and Environmental Microbiology, 83(16), e00953-17. doi:10.1128/AEM.00953-17
Honkinen M., Lahti E., Österback R., Ruuskanen O., Waris M., (2012). Viruses and bacteria in sputum samples of children with community-acquired pneumonia. Clinical Microbiology and Infection, 18(3), 300-307. doi:10.1111/j.1469-0691.2011.03603.x
Gentile A., Bardach A., Ciapponi A., Garcia-Marti S., P Aruj P., Glujovsky D., Calcagno J.I., Mazzoni A., Colindres R.E., (2012). Epidemiology of community-acquired pneumonia in children of Latin America and the Caribbean: a systemic review and meta-analysis. International Journal of Infectious Diseases, 16(1), e5-e15. doi:10.1016/j.ijid.2011.09.013
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