FORENSIC MEDICAL ASSESSMENT OF MORPHOLOGICAL CHANGES AT DIFFERENT POSTMORTEM INTERVAL
PDF

Keywords

forensic medicine, forensic medical examination, postmortem interval, thanatology.

Abstract

Purpose: The postmortem interval (PMI) evaluation is one of priorities while performing a forensic medical examination of corpse. To date, there is lack of information of morphological postmortem changes of some internal organs. Considering the persistent need to develop the method for a precise assessment of PMI, postmortem changes in these potentially informative organs were evaluated. The aim of study was to analyze morphological postmortem changes in prostate and uterus.

Materials and Methods: histological samples of 40 prostate tissues and 40 uterus (n=80) from corpses of deceased aged 18-75 years. Only cases with known time of death were included to study, the time of death was taken from police reports. Exclusion criteria were cases of violent death, cases of death with massive blood loss, tumors of studied internal organs, cases when diagnosis was not made by a forensic medical examiner. The PMI of studied cases ranged from 1 to 6 days. Histological slides were made with a staining by hematoxylin and eosin, x200 magnification, using Olympus ВХ41 and Olympus ВХ46 microscopes, Olympus SC50 camera. Postmortem morphological changes were evaluated by a calculation of blank spaces percentage in microscopical structures using a JS-based program. Connection between PMI and morphological changes was calculated by the Spearman’s rank correlation.

Results: the average percentage of blank spaces in uterus tissues was smaller than in prostate tissues (1,99 and 9,65 relatively). The slower growing of blank spaces was in uterus. In prostate samples, a notable increase of blank spaces was observed between 48 and 72 hours after the death. After this period, the increase slowed down and then an increase was observed again between 120 and 144 hours after the death. In uterus samples, a slight acceleration observed between 72 and 120 hours after the death and then slowing down between 120 and 144 hours after the death. Blank spaces in evaluated histological slides were increasing directly proportional to the PMI, a statistically significant interconnection was defined (p < 0.05).

Conclusions: The morphological postmortem changes in prostate and uterus were developing at certain time frames. Blank spaces percentage, in studied histological slides, were increasing directly proportional to the PMI increase, a statistically significant interconnection was defined. Therefore, the results of study show the possibility of the evaluation of a postmortem time interval by assessing such morphological changes in these organs, which could be used in forensic medical cases.

https://doi.org/10.35339/ic.8.3.177-181
PDF

References

Ciaffi, Romina & Feola, Alessandro & Perfetti, Emilio & Manciocchi, Stefano & Potenza, Saverio & Marella, Gian.. Overview on the estimation of post mortem interval in forensic anthropology: review of the literature and practical experience. Romanian Journal of Legal Medicine, 26, 2018; 403-411.

Hauther KA, Cobaugh KL, Jantz LM, Sparer TE, DeBruyn JM. Estimating time since death from postmortem human gut microbial communities. J Forensic Sci 2015; 60 (5): 1234-1240.

Javan GT, Finley SJ, Can I, Wilkinson JE, Hanson JD, Tarone AM. Human thanatomicrobiome succession and time since death. Sci Rep, 2016; 6:29598.

Belsey SL, Flanagan RJ. Postmortem biochemistry: current applications. J Forensic Leg Med 2016; 41:49-57.

20. Finley SJ, Pechal JL, Benbow ME, Robertson BK, Javan GT. Microbial Signatures of Cadaver Gravesoil During Decomposition. Microb Ecol. 2016; 71 (3): 524-529.

Cobaugh KL, Schaeffer SM, DeBruyn JM. Functional and Structural Succession of Soil Microbial Communities below Decomposing Human Cadavers. PLoS One. 2015; 10 (6): e0130201.

Bugelli V, Forni D, Bassi LA, Di Paolo M, Marra D, Lenzi S, Toni C, Giusiani M, Domenici R, Gherardi M, Vanin S. Forensic entomology and the estimation of the minimum time since death in indoor cases. J Forensic Sci. 2015; 60 (2): 525-531.

Mohr, R.M., Tomberlin, J.K. Development and validation of a new technique for estimating a minimum postmortem interval using adult blow fly (Diptera: Calliphoridae) carcass attendance. Int J Legal Med 2015; 129: 851–859.

Mañas-Jordá S, León-Cortés JL, García-García MD, Caballero U, Infante F. Dipteran Diversity and Ecological Succession on Dead Pigs in Contrasting Mountain Habitats of Chiapas, Mexico. J Med Entomol. 2018 Jan 10;55(1):59-68.

Jarmusz M, Bajerlein D. Decomposition of hanging pig carcasses in a forest habitat of Poland. Forensic Sci Int. 2019 Jul;300:32-42.

Matuszewski S. A general approach for postmortem interval based on uniformly distributed and interconnected qualitative indicators. Int J Legal Med. 2017 May;131(3):877-884

Sutton L, Byrd J. An introduction to postmortem interval estimation in medicolegaldeath investigations. WIREs Forensic Sci. 2020;2:e1373

Holly Lutz, Alexandria Vangelatos, Neil Gottel, Antonio Osculati, Silvia Visona, Sheree J. Finley, Jack A. Gilbert, Gulnaz T. Javan. Effects of Extended Postmortem Interval on Microbial Communities in Organs of the Human Cadaver Front. Microbiol., 2020

Olkhovsky VO, Grygorian EK, Myroshnychenko MS, Kozlov SV, Suloiev KM, Polianskyi AO, Kaplunovskyi PA, Fedulenkova YY, Borzenkova IV. Morphological features of the uterus in women at different time intervals of the postmortem period as diagnostic criteria for establishing the postmortem interval. Wiad Lek. 2021;74(4):821-827.

Uddin, MS & Al-Muhaimin, M & Begum, N & Sultana, Z. (2013). Age Related Changes of Human Uterus-A Postmortem Study. Medicine Today. 24. 10.3329/medtoday.v24i2.15010.

N Abdel Rahman Mahmoud, A Abdel Rahman Abdel Rahman Hassan, A Hassan Abdel Rahim, S Mostafa Mahmoud, O Hassan Nada, Molecular versus histopathological examination of the prostate gland in the estimation of post-mortem interval (an experimental study), QJM: An International Journal of Medicine, Volume 111, Issue suppl_1, December 2018, hcy200.054,

Elgawish, R., Abdelrazek, H., Desouky, A., Mohamed, R. (2021). Determination of postmortem interval through histopathological alterations and collagen evaluation in the prostate of Wistar albino rats. Zagazig Journal of Forensic Medicine, 19(2), 1-12.

Copyright for articles published in the journal is regulated by the License Agreement for the use of a scientific article in the journal, which is concluded between the author of the article (Licensor) and Kharkov National Medical University (Licensee, publisher of the journal "Inter Collegas"). The licensor grants to the Licensee a non-exclusive non-exclusive license for the use of the article (a license that does not exclude the use of the article by the Licensor and the issuance of licenses to others for use of this article) on the terms and for the period specified in the contract. The licensor (the author of the article) grants the Licensee the right to reproduce the article (publication in the journal "Inter Collegas", publication, duplication, duplication or other reproduction of the article without limiting the circulation of copies, each copy of the article must contain the name of the Licensor; Of general information, including the publication of the article in full or in part on the Internet on the journal page, the right to use the metadata of the article (titles, full names of authors, annotations, bibliography eskih materials) through the dissemination and communication to the public, processing and systematization, as well as inclusion in various databases and in-formational system).

The licensor grants the licensee the right to transfer, store and process his personal data (full name, scientific degree, academic title, place of work and position, contact information of the authors) with the purpose of including them in the database in accordance with the Law of Ukraine No. 2297 - VI "on protection of personal data" from 01.06.2010.

Personal data and metadata of the article are provided for their storage and processing in various databases and information systems, including them in analytical and statistical reporting, creating sound relationships between the objects of works of science, literature and art with personal data, etc. on unlimited territory. The licensee has the right to transfer the specified data for processing and storage to third parties provided that such a fact is notified with the provision of information about the third party (name and address) to the Licensor.