How to Cite
Abstract
INDUCED SPUTUM ANALYSIS OF INTERLEUKINS SPECTRUM IN CHILDREN WITH PULMONARY DISEASES
Senatorova A.S., Lupaltsova O.S.
Kharkiv National Medical University, Ukraine
Abstract. This article has been performed the investigation of immunity system characteristics in children with bronchitis. There has been identified factors of unfavourable prognosis of bronchitis transformation in other chronic lung diseases, which include disorder of cytokines-synthesis. A study was undertaken to determine the airway and lung inflammation, by analysing cytokines in the induced sputum from 38 children with the acute bronchitis, 35 patients with the acute pneumonia, 15 children with the chronic lung disease, which had lung fibrosis, and 18 healthy children. Sputum levels of interleukin-4 (IL-4), interleukin-6 (IL-6), interleukin-8 (IL-8) and interleukin-10 (IL-10) in all groups were statistically significantly increased compared with normal controls. The increase in the interleukins concentration in induced sputum of patient with bronchitis and pneumonia we can use in clinical as the risk subgroup of patients with chronic lung diseases.
Key words: bronchitis, pneumonia, interleukin
АНАЛІЗ ІНТЕРЛЕЙКІНОВОГО СПЕКТРУ ІНДУКОВАНОГО МОКРОТИННЯ У ДІТЕЙ ІЗ БРОНХОЛЕГЕНЕВОЮ ПАТОЛОГІЄЮ
Сенаторова Г.С., Лупальцова О.С.
Харківський національний медичний університет, Україна
Резюме. У статті наведені результати дослідження показників імунної системи у дітей з бронхітами. Були відображені фактори трансформації бронхітів в хронічну бронхолегеневу патологію, включаючи порушення продукції цитокінів. Дослідження визначило умови персистенціїї бронхолегеневого запалення, за допомогою вивчення концентрації цитокінів в індукованому мокротинні у 38 дітей з гострими бронхітами, у 35 дітей з пневмоніями, у 15 дітей з хронічною бронхолегеневою патологією та пневмофіброзом, та у 18 здорових дітей. При порівнянні з показниками групи контролю у пацієнтів всіх досліджуваних груп відзначалися статистично достовірно підвищені рівні інтерлейкінів (ІЛ-4, ІЛ-6, ІЛ-8, ІЛ-10) в індукованому мокротинні. Підвищення концентрації інтерлейкінов у індукованому мокротинні у дітей з бронхітами та пневмоніями, можливо використовувати в якості виявлення групи ризику у формуванні хронічної бронхолегеневої патології.
Ключові слова: бронхіт, пневмонія, інтерлейкін
АНАЛИЗ ИНТЕРЛЕЙКИНОВОГО СПЕКТРА ИНДУЦИРОВАННОЙ МОКРОТЫ У ДЕТЕЙ С БРОНХОЛЕГОЧНОЙ ПАТОЛОГИЕЙ
Сенаторова А.С., Лупальцова О.С.
Харьковский национальный медицинский университет, Украина
Резюме. В статье представлены результаты исследования показателей иммунной системы у детей с бронхитами. Были выявлены факторы неблагоприятного прогноза и трансформации бронхитов в хроническую бронхолегочную патологию, включающие нарушение продукции цитокинов. Исследование определило факторы, способствующие персистенции бронхолегочного воспалительного процесса, с помощью изучения концентрации цитокинов в индуцированной мокроте у 38 детей с острыми бронхитами, у 35 детей с пневмониями, у 15 детей с хронической бронхолегочной патологией, имеющих пневмофиброз и у 18 здоровых детей. При сравнении с показателями группы контроля отмечалось достоверное повышение уровней интерлейкинов (ИЛ-4, ИЛ-6, ИЛ-8, ИЛ-10) в индуцированной мокроте во всех исследуемых группах. Повышение концентрации интерлейкинов в индуцированной мокроте у пациентов с бронхитами и пневмониями, можно использовать в качестве выявления группы риска формирования хронической бронхолегочной патологии.
Ключевые слова: бронхит, пневмония, интерлейкин.
References
Strieter R.M. What differentiates normal lung repair and fibrosis? Inflammation, resolution of repair, and fibrosis/ R.M. Strieter // Proceedings of the American Thoracic Society. - 2008. - Vol.5, №3. - P. 305–310.
Mazur W. Сomparison of 8-Isoprostane and Interleukin-8 in Induced Sputum and Exhaled Breath Condensate from Asymptomatic and Symptomatic Smokers/ W. Mazur, H. Stark ,VL. Kinnula // Respiration.- 2009.- Vol.№78. - Р. 209–216.
Induced sputum in children with newly diagnosed mild asthma: the effect of 6 months of treatment with budesonide or disodium cromoglycate / P. Rytila, A.S. Pelkonen, T. Metso [et al.] // Allergy. - 2004.-Vol. 59, № 8. - Р. 839–844.
Weiszhar Z. Induced sputum analysis: step by step / Z.Weiszhar, I. Horvath // Breathe. - 2013.- Vol.9(4).-P. 300-306.
Sagel S.D. Sputum Biomarkers of Inflammation in Cystic Fibrosis Lung Disease/ S.D. Sagel, J.F. Chmiel, M.W. Konstan // Proceedings of the American Thoracic Society.- 2007. – Vol. 4, №4 – P.406-417.
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